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KMID : 1234420100380010084
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Korean Journal of Microbiololgy and Biotechnology
2010 Volume.38 No. 1 p.84 ~ p.92
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Purification and Characterization of Antibacterial Compound Produced by Bacillus subtilis MJP1
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Yim Eun-Jung
Yang Eun-Ju
Chang Hae-Choon
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Abstract
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Antibacterial compound from Bacillus subtilis MJP1 was purified using C18 Sep-Pak cartridge, ion exchange chromatography, and gel filtration chromatography. The purified antibacterial compound showed antibacterial activity against Listeria monocytogenes, Bacillus subtilis, Staphylococcus aureus subsp. aureus, and Enterococcus faecalis. The purified antibacterial compound was found to be stable at 100oC for 5 min and in the pH range of 3.0~9.0, but it was unstable at pH 10.0. It was inactivated by proteinase K and pronase E, and heat treatment at 121oC for 15 min, but it was stable with lipase and α-amylase treatment, which indicated its proteineous nature. Ultra performance liquid chromatography and electrospray ionization tandem mass spectrometry analysis were used to identify the purified antibacterial compound and confirmed the existence of two peptides (3356.54 Da, 3400.5244 Da).
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KEYWORD
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Bacillus subtilis, antibacterial activity, peptide, purification
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